Once in the source, sample molecules are subjected to ionization. Gas chromatography, Liquid chromatography, Capillary electrophoresis, etc.). Samples can be introduced to the mass spectrometer directly via solids probe, or in the case of mixtures, by the intermediary of chromatography device (e.g. Mass analyzer (Quadrupole, TOF, Ion Trap, Magnetic Sector)Дetector (Electron Multiplier, Micro Channel Plates MCPs) Ion source (EI, CI, ESI, APCI, MALDI, etc.) TheВ main components of aВ mass spectrometer are: Mass spectrometry uses an instrument called a mass spectrometer. The mass of a molecule or an ion can be presented in daltons (Da) or kilodaltons (kDa). The actual mass of 12C is 12 daltons, with one dalton is equal to 1.661 10 -24 g. The name given to a particular mass spec technique is usually pointing to the ionization method being used.ДЄtomic and molecular masses are assigned relative to the mass of the carbon isotope, 12C, whose atomic weight is defined as exactly 12. Among the most used soft ionization techniques are Electrospray (ESI) and Matrix Assisted Laser Desorption (MALDI). For samples that are thermolabile such as peptides, proteins and other samples of biological interest, soft ionization techniques are to be considered. For samples that are not themolabile and relatively volatile, ionization such as Electron Impact and/or Chemical Ionization can be effectively used. One of the main factor in choosing which ionization technique to be used is thermolability. Depending on the samples chemical and physical properties, different ionization techniques can be used. The instrument used could be considered as a sophisticated balance which determines with high precision the masses of individual atoms and molecules. Mass spectrometry consists basically of weighing ions in the gas phase. Qualitative and quantitative analysis of components in a mixture. Mass spectrometry is an analytical technique that involves the study in the gas phase of ionized molecules with the aim of one or more of the following: Separation Methods for Coupling with Mass Spec.

Atmospheric Pressure Chemical Ionization.This tutorial discusses basic aspects of mass spectrometry that will be helpful to you in deciding the proper techniques and measurements for your research samples.

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